BioNitroPhos is a microbiological preparation intended to increase soil fertility, facilitate the development of normal soil conditions, increase yield quantity and promote the utilization of stubble fields.

 It contains apathogen fungi, nitrogen-fixing and phosphorus-mobilizing bacteria, polysaccharids, polipeptides, enzymes and other additives. It is a known fact that microorganisms present in the soil play a prominent role in the decomposition of organic substances, mobilization of minerals, forming a thick humus-layer, in the continuous nutrient supply of plants and in the inhibition of phytopathogens. In an area of one hectare, soil contains 3- 15 tons of microorganisms, depending on the conditions. The three most important kinds of microorganisms can be found in the following quantity in an are of one hectare.
Bacteriaapprox. 3t/ha
Fungi approx. 6-8t/ha
Algae approx. 0.03-3t/ha
Soil serves as a natural medium for plants and microorganisms. Under natural circumstances the existance of the one determines the existance of the other. In the past decades we restricted only to the replacement of soil nutrients, without having considered that for a succesful  plant cultivation quantities of microorganisms are also necessary. With its activity aimed at increasing yield results (soil tillage, nutrient replacement, plant protection), mankind has completely disturbed the balance, which had been established for millenia. The environmental burdening of soil has increased and hereby the number and composition of soil bacteria changed.
Agricultural areas have became acidified, waters have been contaminated with nitrates and in plants such substances have appeared, which exert unfavourable effects on wildlife (heavy metals, residues of plant protection chemicals and their metabolits).
The direction of future is the restoration of soil conditions by applying such environment-friendly substances, which increase soil fertility and at the same time, do not endanger the environment and reconstruct the original ecosystem.
Natural soil is a system of complex substances of different quality and physical condition, it is a nutrient medium for plants consisting of 4 mean components:
• Organic substances
• Inorganic components (minerals)
• Soil solution (groundwater)
• Gases (air, carbon-dioxide)
In course of the top-soil formation, complex physical-biochemical processes take place with the participation of soil constituting substances. From the aspect of plant cultivation, the most important conditions to these processes are the chemical elements and the energy, carbon, nitrogen and phosphorus cycle, which is essential to the life functions of cells. BioNitroPhos exerts an effect on the different soil constituting systems, in order that natural processes influence the cycle of nutrients, the fertility, the energy processes and the water balance properties of the soil.

Microorganisms present in BioNitroPhos provide decomposition of organic soil substances, introduction of nitrogen into the soil and mobilization of phosphorus. Our aim is to promote the formation and spreading of environtmental- friendly farming systems besides increasing yield results and improving crop quality with regard to the expectations and regulations of the EU. BioNitroPhos, as a bioproduct, possesses the certification of Bioculture Association and its  production is inspected by Biokontroll Hungária Kht. Soil carbon source is produced predominantly by the utilization of sun energy, by photosynthesis. 50-90% of carbon assimilated hereby ends up in
the soil, gets mineralized and moves to the humus-layer. Humus is the end product of organic decomposition. It consists of very small particles. This formation contains nutrients in the root zone, which are important for plants. Nitrogen, an important element necessary for the growth of plants can be introduced into to soil by means of artificial or natural fertilizers. Nitrogen source supplied by artificial fertilizers can not be entirely utilized, as a part of it gets washed out and another part escapes. The decomposition product of organic
substances of animal or plant origin introduced into the soil, is ammonia. Green plants are unable to take up the nitrogen of ammonia. It
is transformed into nitrites and nitrates by nitrifying bacteria. These ions can be directly utilized by plants. Air is an inexhaustible nitrogen source with a nitrogen content of 78v/v%.
Plants are unable to take up and assimiliate the nitrogen of air. We can provide a continuous nitrogen-supply for our plants with the help of microorganisms present in BioNitroPhos. These are nitrogen-fixing microorganisms and microorganisms changing the structure of soil ammonia.
Hereby 40-60% of artificial fertilizers can be substituted. Soil phosphorus can be found in bound form in organic and inorganic substances. It is mostly insoluble or hardly soluble, in an unabsorbable form for plants. By introducing phosphorus-mobilizing strains present in BioNitroPhos into the soil, after their propagation, complex phophorus-compounds normally unavailable for plants become soluble.
BioNitroPhos is a non-toxic, to humans, animals and to the environment non-hazardous microbiological concentrate of natural origin.
Storage rot causing fungi present in BioNitroPhos are able to produce enzyimes (eg. ligninolytic enzymes, cellulase, hemicellulase, ligninase, lignin-peroxidase, laccase, etc.) necessary to degrade structure substances of plants (lignine, cellulose, hemicellulose). One of the fungal strains possesses a significant lignin-degrading activity, whereas the other one decomposes lignin and cellulose at the same rate. These fungi considerably facilitate the complex degradation of hemicellulose and provide an excellent carbon and energy source for cultivated plants and soil bacteria fixing molecular nitrogen and mobilizing phosphorus. Fungi present in the preparation are apathogen and do not infect living plants. One of the nitrogen- fixing bacterium strains of BioNitroPhos lives normally in the soil.
This microorganism is able to fix themolecular nitrogen of air, and to reduce it into ammonia with the help of an electron mediator molecule and a nitrogenase enzyme. Ammonia is transformed by nitrifying bacteria into nitrites and nitrates, which is easily absorbable
for plants.
It is able to fix as much as 20-30 kg nitrogen per hectare from the air during one plant cultivation cycle. The other nitrogen-fixing bacterium strain is able to fix nitrogen in the proximity of another pre-organism, eg. root-system of a plant. In addition to providing the necessary nitrogen for the growth of plants, its importance lies also in the fact that it is able to produce hormones, substances stimulating the growth of plants.
It is able to fix as much as 40-60 kg nitrogen per hectare during one plant cultivation cycle. The phosphorus-mobilizing bacterium strain of BioNitroPhos plays a role in iron absorbation at the same time. Under natural conditions, phosphorus can be found in organic and inorganic compounds of the soil. These phosphorus-compounds are hardly soluble or insoluble in water. Their availability for plants is minimal. By introducing and propagating phosphorus-mobilizing microoganisms into the soil, complex phosphorus-compounds normally not available for plants become soluble.
In addition, the persence of phosphorus-mobilizing microorganisms has an important role, as they are able to capture iron also if low iron
concentration is present, and to form so-called sideophores.
The result thereof is an inhibition of harmful bacteria and fungi growing in the rhizosphere eg. propagation inhibition of the potato pathogen Ervinia caratovora by means of iron-deficiency, or growth stimulation of potato, sugar beet and radish on an iron deficient soil by delivering captured iron directly for the cultivated plants.
It has a great importance from the aspect of plant cultivation that phosphorus-mobilizing bacteria are able to synthetize hormones stimulating the development of plants (eg. gibberelin acid).

Advantages of the application of BioNitroPhos

Effects exerted by fungi
• Facilitates quick decomposition of organic substances and formation of humus
• Supersedes stubble burning and hereby stops the related environment destruction and pollution
• Enables the decompositon of lignin and cellulose containing organic substances ploughed into the soil

Effects exerted by bacteria
• Reduces the needed amount of chemical fertilizers to an economic extent and at the same time, increases their efficiency
and decreases the loss
• Inhibits the propagation of other pathogen bacteria and fungi growing in the rhizosphere
• By fixing nitrogen from the environment (air), reduces the amount of other substances of nitrogen content necessary to introduce into the soil

Joint effects
• Improves soil structure and water balance and hereby reduces soil crustation
• Favourably influences the pollution of surface and groundwaters
• Favourable influences the quantity and structure of the root system
• Under appropriate conditions, in addition to improving fruit quality, it increases crop capacity

Recommended also for biocultivation

RECOMMENDED USE
BioNitroPhos increases soil fertility, and can be applied for all kinds of plant culture.

It can be applied:
• In field vegetable production
• For treating meadow-lands, pastures, grasslands
• In horticulture
• In viticulture
• In tree and forest plantation
• In the cultivation of flowers and ornamental plants
• In top-dressing

Conditions of spreading:
• Windless weather
• Preferably wet soil
• Temperature between 10-25 °C Quick and favourable results can be expected, if the soil meets the following requirements:
• pH value is between 6-7.5
• Organic soil content exceeds 1.5%
• Carbon-nitrogen ratio (C/N) is 12:25

If the soil does not meet these requirements, it is advisable to perform soil conditioning first.
BioNitroPhos may also be applied in case the organic soil content does not reach the 1.5%. In this case 35-40 kg substance of nitrogen content shall be spread into the soil, per hectare.
Time of spreding:
• 2-3 weeks previous to sowing, planting or transplant planting
• In case of grape vine, 2-3 weeks previous to sprouting
• In case of fruit cultivation, after fruit harvest
• 2 weeks before tree or forest plantation
• 2 weeks before planting flowers or ornamental plants
Until restoration of normal soil conditions regular use is extremely important, thereafter it is recommended.

Manner of spreading

The water dilution of BioNitroPhos, or its solution prepared with 35-40 kg inorganic substance of nitrogen content (if it is needed because organic soil content does not reach the 1.5%) shall be evenly spread into the soil by
• Irrigation or
• Spraying
and superficially ploughed into the soil.
BioNitroPhos may be spread into the soil at any time, however, expected results will manifest only after the propagation of microorganisms.

Storage conditions
at 4-5°C 6-8 weeks
at 6-8°C 2-3 weeks
at 10-12°C 6-8 days
The product is a bacteria and fungi concentrate containing living organisms in a great number, so its preservation period depends primarily on the temperature. It can be stored together with other materials in cellars, as it is not dangerous to the environment.
Because of its high organic and microorganism content, temperature of BioNitroPhos may not permanently exceed 15°C during transport and storage.


Recommended quantities for application

By the application of BioNitroPhos following shall be taken into consideration:
• PH value of the soil
• Organic soil content
• Amount of precipitation
• Soil type
• Growing season of the plant
Depending on soil pH: pH=6.5-7.5 10L/ha It soil pH deviates in either direction from this range, quantity shall be increased by 1 litre per 0.5 pH unit.
Depending on organic soil content: 2%, 12 L/ha
If organic soil content rises by 1%, 1 litre less
material shall be spread.
Depending on the amount of precipitation: on wet areas: 10 L/ha On rainless areas: 12 L/ha
Depending on soil type
• Clay soil 11 L/ha
• Sandy soil 13 L/ha
• Adobe soil 10 L/ha
Depending on the plant’s vegetation period:
In case of cultures of a longer vegetation period, it advisable to apply the product also for top-dressing or side-dressing.
BioNitroPhos quantity recommended for application shall be diluted with water to 300-500 litres and spread into the soil.
Experiences with the application of BIONITROPHOS
According to the experiences of users, spreading with BNP technology shortens the period of time necessary to the decomposition of
stubbles, stem and root residues. It also showed to be effective in the decomposition of vineshoot and reed residues.
Considerable sum of money can be saved by the users, because conventional nitrogenous fertilizers are not needed any more to the
decomposition of stem residues.
If applied for stubbles after reaping or for sunflowers or maize in autumn, it promotes the development of appropriate soil quality necessary to the preparation of seed-beds.
In spring, sreading of BNP takes place simultaneously with soil preparation, so the costs of spreading can be minimalized.
On the basis of experiences obtained during years, due to the favourable effects of BNP 510q more yield quantity can be achieved by
cereals, but in an extremely rainless period of time a yield increase of even 70-80% could be measured by sunflowers, compared to the control plants.
Besides cereals, BNP produced a significantly high yield average in case of sugar beet and soy bean. These plants’ average of 35-41 q/ha rose by far above the average of untreated areas, which amounted to 32 q/ha.
In addition to surplus yield, producers always emphasise the improvement of soil quality, which makes further soil farming easier and
saves work.
If applying for field vegetable production, experiences are very favourable with fruit cultivation, in particular with berries.